expression analyses of endoglucanase gene in penicillium oxalicum and trichoderma viride

in this paper detection of endoglucanase gene and protein profile belonging to two fungal species, penicillium oxalicum 1sms and trichodermaviride156ms with high cellulotic activity was investigated. fungi isolates were cultured on inducer cmc medium and then released sugar and protein were assayed every three days for a month using arsenate molybdate reagent and bradford method, respectively. detection of endoglucanase gene was performed by using specific primers. expressed cdna fragment in two isolates revealed a size of 1380 bp to 1434 bp lenght. cdna sequences were sequenced and then were queried in blastn and tblastx search against eg1 genes from other fungi. theses sequences showed 98% homology to eg1 gene deposited in databases. sds-page pattern of protein separation revealed one protein band for each isolate having a molecular weight of 49.786 and 48.339 kda with 487 and 460 amino acids. bioinformatics analysis showed glycosyl hydrolase domains, a cellulase banding domain and 6 prosites for both proteins. one orf in each gene region for each species was determined. the results indicated that carboxy methyl cellulose is able to induce expression of eng1 following endogluconase enzyme production